Coding
PhyB-908-(

Part:BBa_K365011:Design

Designed by: RENAULT Renaud   Group: iGEM10_ESBS-Strasbourg   (2010-09-15)


Phytochrome B (aa 1-908) linked to a trimer of ClpX (aa 61-425)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
    Illegal BglII site found at 3495
    Illegal BglII site found at 4659
    Illegal BglII site found at 5823
    Illegal BamHI site found at 572
    Illegal XhoI site found at 523
    Illegal XhoI site found at 542
    Illegal XhoI site found at 2677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2062
    Illegal BsaI site found at 2468
    Illegal SapI site found at 739


Design Notes

We used Freiburg fusion standard (RFC-25) for in frame assembly.


Source

See subparts.

References